Tools to help you designing your experiment with our BD LSRII

Two sites that display spectral properties of many fluorochromes:
Link to Invitrogen Fluorescence Spectra Viewer
Link to BD Fluorescence Spectrum Viewer

Our solution

It is a simple, but still efficient solution. It will help you to check whether the combination that you intend to use is correct. It works online, just follow the following steps:

1) Select the fluorochrome that you intend to use
2) Fill the 'Antibody' field (for instance Anti-mCD3)
3) If you are able to enter your combination, this means that your combination is correct, simple, isn't it?

Last update: Pacific Orange was added (8 March 2008)

N°
Lasers
Antibody
Fluorochrome
Band Pass
Detector A
Blue (488nm)
780/60
Detector B
Blue (488nm)
720/40
Detector C
Blue (488nm)
685/15
Detector D
Blue (488nm)
660/20
Detector E
Blue (488nm)
610/20
Detector F
Blue (488nm)
575/26
Detector G
Blue (488nm)
530/30
Detector A
Red (633nm)
780/60
Detector B
Red (633nm)
720/40
Detector C
Red (633nm)
660/20
Detector A
Violet (405nm)
790/20
Detector B
Violet (405nm)
695/40
Detector C
Violet (405nm)
655/8
Detector D
Violet (405nm)
605/12
Detector E
Violet (405nm)
550/40
Detector F
Violet (405nm)
500/30
Detector G
Violet (405nm)
450/50


LaserBleu

LaserRouge

LaserViolet