Tools to help you designing your experiment with our BD ACCURI C6

Two sites that display spectral properties of many fluorochromes:
Link to Invitrogen Fluorescence Spectra Viewer
Link to BD Fluorescence Spectrum Viewer


Our solution

It is a simple, but still efficient solution. It will help you to check whether the combination that you intend to use is correct. It works online, just follow the following steps:

1) Select the fluorochrome that you intend to use
2) Fill the 'Antibody' field (for instance Anti-mCD3)
3) If you are able to enter your combination, this means that your combination is correct, simple, isn't it?

 

!!!!!VERY IMPORTANT!!!!

Please note that the C6 can have 3 distinct configurations.

Before starting, you MUST check which filters are installed.

If you change the default configuration (*), you MUST put it back when you have finished your experiment.


Configuration 3 blue-1 red

Lasers
Antibody
Fluorochrome
Band Pass
Detector FL1
Blue (488nm)
533/30 (*)
Detector FL2
Blue (488nm)
585/40 (*)
Detector FL3
Blue (488nm)

670LP (*)

610/20

Detector FL4
Red (633nm)
675/25 (*)

 

 

Configuration 2 blue-2 red

Lasers
Antibody
Fluorochrome
Band Pass
Detector FL1
Blue (488nm)
533/30
Detector FL2
Blue (488nm)
585/40
Detector FL3/FL4
Red (633nm)
780/60
Detector FL3FL4
Red (633nm)
675/25

 

 

Configuration 4 blue-2 red

Lasers
Antibody
Fluorochrome
Band Pass
Detector FL1
Blue (488nm)
533/30
Detector FL2
Blue (488nm)
585/40
Detector FL3
Blue (488nm)
675/25
Detector FL4
Blue (488nm)

610/20

630/30

780/60