Tools to help you designing your experiment with our BD ACCURI C6
Two sites that display spectral properties of many fluorochromes:
Link to Invitrogen Fluorescence Spectra Viewer
Link to BD Fluorescence Spectrum Viewer
It is a simple, but still efficient solution. It will help you to check whether the combination that you intend to use is correct. It works online, just follow the following steps:
1) Select the fluorochrome that you intend to use
2) Fill the 'Antibody' field (for instance Anti-mCD3)
3) If you are able to enter your combination, this means that your combination is correct, simple, isn't it?
Please note that the C6 can have 3 distinct configurations.
Before starting, you MUST check which filters are installed.
If you change the default configuration (*), you MUST put it back when you have finished your experiment.
Configuration 3 blue-1 red