Magnetic nanoparticles: new opportunities for advanced in vitro diagnostics and biomedical research in vivo


Petr NIKITIN. General Physics Institute, Russian Academy of Sciences, Moscow.

Modérateur : Soraya LANOUAR


Several recent original developments will be overviewed such as:


  1. Synthesis of several types of magnetic nanoparticles (MP) for analytical and biomedical applications (for labeling of drugs and biomolecules, MRI contrasting, etc.).
  2. Ultra-sensitive methods for magnetic nanoparticle quantification (MPQ) by non-linear magnetization for in vitro and in vivo applications (current multiplex MPQ readers provide detection limit of 60 zeptomoles or 0.4 ng of MP and extremely wide linear dynamic range of 7 orders).
  3. Multiplex biosensing and cytometry systems for in vitro diagnostics by using MP as labels in immunoassays (e.g., for rapid detection of PSA at 25 pg/ml and 3 order dynamic range, for rapid assessment of cancer HER2/neu status of cells and cancer diagnostics, etc.).
  4. Various aspects of in vivo studies of MP in laboratory animals (dynamics of MP in blood flow, distribution in organs, biodegradations, etc.).
  5. Development of “biocomputing” or “intelligent” theranostic agents that can autonomously act in a pre-programmed way under different factors (e.g., presence of specific molecules in a local medium, pH, etc.)
  6. Investigation of the biocomputing theranostic agents based on the localized plasmon resonance with gold and magnetic nanoparticles.


Recent publications:

  1. Nikitin et al. Biocomputing based on particle disassembly. Nature Nanotechnology. 9 (2014) 716–722.
  2. Orlov et al. Rapid dry-reagent immunomagnetic biosensing platform based on volumetric detection of nanoparticles on 3D structures. Biosensors and Bioelectronics. 79 (2016) 423–429
  3. Orlov et al. Multiplex biosensing based on highly sensitive magnetic nanolabel quantification: rapid detection of botulinum neurotoxins A, B and E in liquids. Analytical Chemistry. 88 (2016) 10419−10426.
  4. Shipunova et al. MPQ-Cytometry: a magnetism-based method for quantification of nanoparticle-cell interactions. Nanoscale. 8 (2016) 12764-12772.
  5. Morozov et al. Generation and Delivery of Nanoaerosols from Biological and Biologically Active Substances. Journal of Aerosol Science. 69 (2014) 48-61.
  6. Shevchenko et al. Surface plasmon resonance as a tool for investigation of non-covalent nanoparticle interactions in heterogeneous self-assembly & disassembly systems. Biosensors & Bioelectronics. 88. (2017) 3–8.